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Shorthand for mass spectrometry, the analytical method used to confirm peptide molecular identity by measuring precise molecular weight.
Mass spec is the common laboratory abbreviation for mass spectrometry, the analytical technique that identifies compounds by measuring the mass-to-charge ratio of their ionised molecules. In peptide quality control, mass spec serves a complementary role to HPLC: while HPLC quantifies purity, mass spectrometry confirms identity by verifying that the peptide has the correct molecular weight. Common techniques include electrospray ionisation (ESI-MS) for peptide solutions and MALDI-TOF for rapid molecular weight determination. A peptide that passes both HPLC (>99% purity) and mass spec (correct molecular weight match) verification has been confirmed to be both pure and correctly identified. ORYN includes mass spec molecular weight confirmation on every Certificate of Analysis.
TESTING & QC
General research term
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An analytical technique that measures the mass-to-charge ratio of ions to identify and quantify molecules, essential for verifying peptide identity and purity.
High-Performance Liquid Chromatography, the gold-standard analytical method for determining peptide purity by separating and quantifying components in a liquid sample.
A document from a quality testing laboratory that confirms a peptide batch meets specified purity, identity, and quality standards.
Analytical procedures that determine the percentage of desired peptide in a sample versus impurities, contaminants, or degradation products.
A laboratory technique for separating mixture components based on differential interactions with a stationary and mobile phase, used extensively in peptide analysis.
A quality control assay that detects bacterial lipopolysaccharide contamination in injectable peptide products to ensure safety.
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